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>> Large-Scale Protein Production using Hollow Fiber Bioreactors >> Protein Purification >> Protein Conjugation


Large-Scale Protein Production using Hollow Fiber Bioreactors


Hollow Fiber Bioreactors (HFB) are used primarily for growing antibody secreting hybridomas and transfectomas at high densities to achieve high-titer antibody containing supernatent.

Mammalian cell cultures, for years, have been grown in 2-dimensional, non-porous, culture plates. These plates limit cell growth by needing to be split or passed and given fresh media every few days. After about 2 days, the cells have nearly consumed all the nutrients and are bathing in their waste. This feast and famine routine inhibits cells full potential as far a growth rates and antibody/protein secretion rates. The Hollow-Fiber Bioreactor technology addresses all these concerns.

Hollow Fiber Bioreactors allow for:

  • Easy scale-up of cells without having stacks of flasks fill up the incubator
  • Increase antibody titer up to 100 fold from static culture flask supe.
  • 3-D growth of cells up to tissue-like densities supported by porous fiber matrix.
  • Reduced cell toxicity from waste and pH changes.
  • Less overall maintainance of cell culture
  • Cost savings for production of greater than 50mg of protein.

For more information regarding this technology, please contact the facility.


A hollow-fiber bioreactor

 

 
University of Chicago Biological Sciences Division Shared Research Facilities Core