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>> Antibody/Hybridoma Bank >> FAQs

PLEASE NOTE THAT THE BELOW LISTED ANTIBODIES ARE AVAILABLE ONLY TO
University of Chicago INVESTIGATORS.

ALL ANTIBODIES ARE AVAILABLE FOR PURCHASE FROM COMMERCIAL VENDORS.

Please contact Thelma Tennant (ttennant@tech.uchicago.edu; 773-834-4020) if you would like a list of commercial vendors or if you are interested in licensing any of these monoclonal antibodies.

 

Click HERE to download your own copy of our Antibody/Hybridoma Bank offerings.

 

Antibody/Hybridoma Bank

Hybridomas (Available as frozen stocks, supernatant or purified.)

Against Cell Surface Molecules
Name
Reported Applications
Isotype
Possible Isotype Control
MAP Testing Exempt
MAP (-)
Murine
 
 
 
Rat anti-mouse CD4
GK1.5
IF, IP, W
IgG2b
Rat anti-mouse CD8
2.43.1
FC
IgG2b
Rat anti-mouse CD8 (Lyt2.2)
3.155
FC
IgM
Rat anti-rat Lyt-2 (anti-CD8)
YTS-169.4.2.1
FC
IgG2b
Rat anti-mouse Fcγ III/II
2.4G2.1
FC
IgG2b
Armenian Hamster anti-mouse CD3e
145-2C11.1
FC, IHC
IgG
Armenian Hamster anti-mouse CD1.1
F7-5C6.4.18
 
IgG
 
Armenian Hamster anti-mouse CD28
PV-1
FC, IP
IgG
 
Mouse anti-mouse NK1.1
PK136
FC
IgG2a
Rat anti-mouse Stem cell antigen 1
Sca1
IF, IP
IgG2a
Rat anti-mouse CD19
1D3
IF
IgG2a
Rat anti-mouse CD62L (L-selectin)
MEL-14
FC, IHC, IF
IgG2a
Hamster anti-mouse CTLA4
1B8
FC
IgG
Hamster anti-mouse CTLA4 (anti-CD152)
UC10-4F10
FC, IP
IgG
Hamster anti-mouse TCR gamma/delta
UC7-13D5
FC
IgG
Rat anti-mouse IL2 receptor alpha
PC61-5-3
FC
IgGl
Rat anti-mouse Tcell recep. of 2C transg. mice
1B2
FC
IgGl
Hamster anti-mouse B7-1 (anti mouse CD80)
16-10A1
FC
IgG
Rat anti-mouse Ly-6G (GR-1)
RB6-8C5
FC
IgG2b
Anti-mouse phosphorylcholine
BH8
IHC
IgM
Anti-mouse T15 idiotype
AB1-2
IgGl
Rat anti-mouse F4/80(macrophage)
F4/80
FC, W, IF, IP
IgG2b
Rat anti-mouse CD45R/B220
RA3-6B2
FC, IF
IgG2b
Mouse anti-mouse CD45.1
A20
FC, IF, IP
IgG2a
Rat anti Thy1.2
AT83A
CD
IgM
Rat anti-mouse GPI light chain (glucose-6-phosohate isomerase) 6.121
F156-6B9.7
IgG2a
Mouse anti-hepatocyte growth factor receptor
5D5.11.6
 
IgGl
 
Mouse anti-mouse RAF-1
URP26K
IP,W,E
IgG
     
Rat anti-mouse IL10r
1B1-3a
 
IgGl
     
Mouse anti-mouse EGF receptor
528
IP, W
IgG2a
 
Mouse anti-human CD29, VLA1 receptor
TS2/16.2.1
W
IgGI
 
Mouse anti-human Integrin Alpha V subunit
L230
IP, IHC, IF, FC
IgGl
 
Mouse anti-mouse H-2d, PC.1
P1.17
 
IgG2a
 
 
Human
 
 
 
Mouse anti-human CD3
OKT3
FC
IgG2a
Mouse anti-human CD38
OKT 10
IP, IF
IgG1
Mouse anti-human CD2
OKT 11
FC, WB
IgG1
Mouse anti-human CD1a
OKT6
FC, IP, IHC
IgG1
Mouse anti-human Tcell subsets
OKT5
CD
IgG1
Mouse anti-human CD8
OKT8
FC
IgG2a
Mouse anti-human MHC Class I(anti-HLA-ABC)
W6/32
WB,IF,FC
IgG2a
 
Against Intracellular Molecules
Rat anti-mouse IFN γ
XMG1.2.20
FC
IgGl
Rat anti-mouse IFN γ
R46A2
IgGl
     
Rat anti-human estrogen receptor alpha
H222.2
W, IF, IHC, IP
IgGl
Rat anti-human estrogen receptor alpha
D75
W, IHC, IF
IgG2a
 
Rat anti-human progesterone receptor
KD68
W
IgG2a
 
Mouse anti-human & rodent Inosine monophosphate dehydrogenase (IMPDH)
1D6-E3
W, IF
IgGl
 
Against nominal Proteins and Protein Tags
Mouse anti-OVA
F2-3.58
IgGl
Mouse anti-GFP
F56-6A1.2.3
W
IgG2b
Mouse anti-mouse cMyc
9E10
W, FC, IF, IHC
IgGl
Mouse anti- HA
12CA5
W, IP, IF
IgG2b
Mouse anti-GST
F50-3D12.2
W
IgGl
 
 
Cell lines
WEHI 231
mouse B cell Lymphoma (Balb/c x NZB)F1
BHK
baby Syrian hamster kidney
EL4
mouse (BI/6) Lymphoma H2bThyl1.2
P815
mouse (DBA/2) mastocytoma (transfection host)
Jurkat
human male T lymphocyte (acute T cell leukemia)(transfection host)
Myeloma fusion partners ( Available only as frozen stocks)
Sp2/0
mIl6SP2/0 (Spent supernatant containing 3.9 μg/ml mIL6 also available)
P3x63AG8.653
 

Available as 100 ml supernatant ($64), or purified Ab ($40/mg)

 

All hybridomas are ELISA positive.

 

All antibodies can be conjugated to FITC or Biotin in 24 hrs.

 

Biotinylated RB6-8C5 (GR-1) titered to 1:1600 on LSR Fortessa.


FC  Flow cytometry, W  Western blot, IHC  Immunohistochemistry, IP-Immunoprecipitation, CD-Cell Depletion,IF-Immunnofluoresence


Please contact Mandel Davis, Kovler 055, 702-7293 or mdavis4@bsd.uchicago.edu for additional information or questions.


Click HERE to download your own copy of our Antibody/Hybridoma Bank offerings.